Recto seeks to establish Taal rehab body to aid community, eruption victims Cabuyao City rising above the ashes through volunteerism Lights inside SMX hall flicker as Duterte rants vs Ayala, Pangilinan anew Sevilla also lost at home in the night’s other quarterfinal, as Bayern Munich came from behind to win 2-1. Jesus Navas’ own goal cancelled out Pablo Sarabia’s opener and Thiago Alcantara headed in the winner for Bayern in the 68th minute.In Turin, Juventus was out for revenge after losing the final 4-1 to Madrid last year. But Ronaldo once again came out on top.The Portuguese forward has scored in every Champions League match this season and it took him less than three minutes to continue that tradition, thanks in part to a series of uncharacteristic defensive mistakes from Juventus.Isco was left in acres of space on the left flank and he crossed in for an unmarked Ronaldo to poke the ball into the right side of the net.Juventus responded well and could have leveled immediately but Sergio Ramos put in a perfectly-timed tackle just as Paulo Dybala was about to unleash a shot.Ramos was on hand again in the 13th minute to head Rodrigo Bentacur’s effort away near the goalline.Juventus — and most of the stadium — thought it had equalized midway through the half but Madrid goalkeeper Keylor Navas pulled off a stunning save to deny Gonzalo Higuain after Dybala whipped in a free kick from the left.Having got the away goal, Real Madrid was seemingly content to sit back and hit Juventus on the counter and the Spanish side almost doubled its lead 10 minutes from the break when Toni Kroos’ fierce effort crashed off the crossbar from 25 yards.Juventus had two penalty appeals waved away on the stroke of halftime as referee Cuneyt Cakir ruled that Casemiro’s handball was not deliberate and moments later adjudged that the same player had not fouled Dybala and instead booked the Argentina international for simulation.Both sides continued to have chances after the break. Ronaldo’s angled drive flashed just past the left post, while Dybala’s free kick was deflected narrowly over the bar with Navas wrong-footed. Juventus goalkeeper Gianluigi Buffon had just pulled off a fantastic save to deny Lucas Vazquez but the move continued and Ronaldo leaped acrobatically into the air to meet a cross with his back to goal and smashed the ball into the bottom right corner.Even the Juventus fans rose to give him an ovation, having jeered his every touch previously. And Madrid coach Zinedine Zidane, himself considered one of the best players in history, clutched his head in amazement.FEATURED STORIESSPORTSGinebra beats Meralco again to capture PBA Governors’ Cup titleSPORTSAfter winning title, time for LA Tenorio to give back to Batangas folkSPORTSTim Cone, Ginebra set their sights on elusive All-Filipino crown“Cristiano is simply a different player than all the others,” Zidane said. “He shows that every time. He always has a great desire to do great things in the Champions League, he never gets tired of doing what he does. … I’m the coach but also a fan. That’s why I put my hands on my head.”Ronaldo had also scored the opening goal just three minutes into the game, and he later set up the third for Marcelo as Madrid took a commanding lead back home for the second leg. Don’t miss out on the latest news and information. In the process, the Portuguese star became the first player in Champions League history to score in 10 successive matches.“There are two teams better than anyone and they are those that have (Lionel) Messi and Ronaldo,” Juventus coach Massimiliano Allegri said. “They have won 10 Ballons d’Or in 10 years, they have been the Champions League’s top goalscorers for 10 years.“For that reason Real Madrid and Barcelona have the advantage.”Juventus now has a near-impossible task to turn the quarterfinal around against the two-time defending champions in the return leg on April 11, after falling to their first home defeat in the Champions League since April 2013.“You never know in football,” Allegri said. “We have to prepare it in the best way possible because it’s a quarterfinal and everyone will be watching us.”ADVERTISEMENT View comments Mavs’ Noel, Jazz’s Sefolosha get 5-game drug bans from NBA Nadine Lustre’s phone stolen in Brazil MOST READ Scarlett Johansson, Sterling K. Brown among SAG Awards presenters In Liverpool, Man United sees the pain and path to recovery Ramos was booked for the challenge that led to that free kick and will be suspended for the second leg.The match changed shortly after the hour mark.Gianluigi Buffon pulled off a fantastic save to deny Lucas Vazquez but the move continued and Ronaldo leaped acrobatically into the air with his back to goal and smashed the ball into the bottom right corner.The goal was so good that even the Juventus fans rose to applaud — having jeered his every touch previously.They were still on their feet moments later when Dybala was shown a second yellow card for a foul on Dani Carvajal.Ronaldo turned provider in the 73rd minute as he played a one-two with Marcelo before the Brazil defender lifted the ball over Buffon and bundled it into the back of the net.It could have been worse for Juventus as Ronaldo missed several chances to complete his hat trick, while Mateo Kovacic hit the bar late on.Sports Related Videospowered by AdSparcRead Next Judy Ann’s 1st project for 2020 is giving her a ‘stomachache’ Carpio hits red carpet treatment for China Coast Guard PLAY LIST 02:14Carpio hits red carpet treatment for China Coast Guard02:56NCRPO pledges to donate P3.5 million to victims of Taal eruption00:56Heavy rain brings some relief in Australia02:37Calm moments allow Taal folks some respite03:23Negosyo sa Tagaytay City, bagsak sa pag-aalboroto ng Bulkang Taal01:13Christian Standhardinger wins PBA Best Player award Conor McGregor seeks to emerge from controversy in UFC comeback Jiro Manio arrested for stabbing man in Marikina Real Madrid’s Cristiano Ronaldo celebrates after scoring the second goal of his team during the Champions League, round of 8, first-leg soccer match between Juventus and Real Madrid at the Allianz stadium in Turin, Italy, Tuesday, April 3, 2018. (AP Photo/Luca Bruno)TURIN, Italy — Cristiano Ronaldo latest masterpiece was so good even the opposing fans had to stand up and applaud.Ronaldo pulled off a spectacular overhead kick to score his second goal of the night on Tuesday as his Real Madrid beat Juventus 3-0 in the first leg of their Champions League quarterfinal. Even by Ronaldo’s record-breaking standards, this one was special.ADVERTISEMENT LATEST STORIES
zoomIllustration; Source: PxHere under CC0 Creative Commons license Abu Dhabi Ports has waived charges for over 75% of services offered by its subsidiary Khalifa Industrial Zone Abu Dhabi (KIZAD).As explained, the move is in line with the Abu Dhabi Government directive to encourage further investment into the emirate.Specifically, three quarters of services will be offered free of charge, while fees for a significant number of the remaining services will also be reduced and streamlined to a simpler tariff structure. “We continue to support the government initiatives to build an investor-friendly environment by providing our partners and customers with the incentives they need for their businesses to thrive and grow. We are committed to Abu Dhabi’s drive to become a global gateway for businesses from all parts of the world,” Captain Mohamed Juma Al Shamisi, CEO of Abu Dhabi Ports, commented.“The cost of setting up and maintaining a successful business at KIZAD is more achievable now than ever before. It is an ideal opportunity for companies of all sizes to benefit from such incentives for either growing their business or entering new markets.”The exemptions are said to be aligned with Ghadan 21, the three-year, AED 50 billion (USD 13.6 billion) Development Accelerator Programme for the emirate.According to Abu Dhabi Ports, the decision to waive fees also builds on the success that the port developer and operator has had in attracting investors to KIZAD since it was launched in 2010. To date, the zone has attracted more than 500 investors and more than AED 65 billion in investment across multiple sectors.The new fee structure will be applicable from this month onwards.Separately, Abu Dhabi Ports said that it and Jiangsu Overseas Cooperation Investment Co. Ltd. (JOCIC) have signed a five-year agreement with Industrial and Commercial Bank of China (ICBC) to make it easier and quicker for Chinese companies to do business at KIZAD.Abu Dhabi Ports and KIZAD have already established with JOCIC a 2.2 square kilometer China-UAE Industrial Capacity Cooperation Demonstration Zone at KIZAD under a 50-year agreement. The Demonstration Zone forms part of the UAE’s support for China’s Belt and Road Initiative (BRI) and aims to attract more trade and investment to Abu Dhabi. The agreement also reiterates the strategic position played by the emirate to the major trade hubs along the BRI.
At least 37 country governments, 16 private companies and 11 partner organizations, including civil society and private foundations, will announce commitments to accelerate progress on rights-based family planning programmes today at the Family Planning Summit.The Family Planning Summit is co-hosted by the UK Government, UNFPA, the United Nations Population Fund and the Bill and Melinda Gates Foundation, in close partnership with the Family Planning 2020 Secretariat (FP2020). In parallel with the Summit in London, more than 2500 people will gather at 21 satellite events across Cameroon, Côte d’Ivoire, Ethiopia, Ghana, Kenya, Indonesia, Malawi, Nepal, Nigeria, Pakistan, Sierra Leone, Thailand and Uganda, demonstrating growing country leadership and support for family planning.The Summit will build on progress made since the Family Planning Summit in 2012 and will reaffirm international political support for women and girls to be able to decide for themselves whether, when and how many children to have. It will address barriers to progress that are common across many countries, with a wide range of policy and programme commitments to ensure the money invested in family planning goes further.Financial commitments to be announced at the Summit are expected to total at least $2.5billion USD (£1.9billion GBP) by 2020. The majority of the funding – $1.5billion USD (£1.16billion GBP) – has been committed by countries in Asia and Africa. Many of FP2020’s 38 partner countries, ranging from Bangladesh and Burkina Faso to India, Indonesia and Uganda, will make renewed commitments to accelerate family planning progress, and four new countries will join the FP2020 partnership.The full breadth of expected Summit commitments from country governments, donors, private sector, civil society and foundations can be found here.Commitments will seek to deliver family planning to all who need it, especially adolescents, women and girls in humanitarian crises and the hard to reach. Highlights of the commitments made include: • A Bridge Funding Mechanism to enable UNFPA Supplies, the world’s largest provider of contraceptives, to provide countries with the family planning commodities they need as efficiently as possible. The Funding Mechanism is expected to speed up the procurement process, lower the cost of contraceptives, and ultimately reduce up to 50 per cent of UNFPA-related contraceptive stock-outs-delivering better results for countries, donors, and the women and families they serve. • A supply chain Visibility Analytics Network (VAN), a global platform to capture and use data from multiple sources to provide enhanced visibility into contraceptive supply chains. By partnering with private sector logistics experts and using smart-phone technology, the network will improve countries’ ability to know what contraceptives are in stock, where and how much – and to respond to stock-outs more efficiently. In two years’ time, we anticipate that this Network will be up and running in at least 12 countries. • Countries, donors, civil society and private foundations will pledge to collect, use, and disseminate age- and sex-disaggregated data. With improved data, governments, charities and others will be better able to design and assess programmes that more effectively meet the needs of adolescents. • Ten private sector organisations from outside the health and pharmaceutical sector, including the Chaudhary Foundation, Twinings, Tata Trusts, Spark Minda, MTV Staying Alive Foundation, Lindex, Vodafone, CARD MRI, NST and Reckitt Benckiser, will make commitments that expand access to contraceptives, improve supply chains, or market and deliver family planning services to their employees. These will include commitments to reach more women and girls, including through their networks, assets, and expertise-from supply chains to microfinance networks to media channels.The Family Planning Summit takes place on the five-year anniversary of the inaugural London Summit on Family Planning when world leaders set a goal to empower 120 million additional women and girls in the 69 lowest income countries to use modern contraception by 2020, and a new partnership was launched: Family Planning 2020 (FP2020). Since then, 30 million additional women and girls have begun using modern contraceptives – bringing the total to a record 300 million users across the 69 countries. As a result, last year more than 82 million pregnancies, 25 million unsafe abortions, and 125,000 maternal deaths were averted.The Summit will feature commentary by government representatives from around the world, the United Nations, foundations, the private sector, civil society and youth advocates from eight countries.Priti Patel, UK International Development Secretary, said: “Family planning has the power to change a whole country’s trajectory and help lift people out of grinding poverty. It gives women choice over if, and when, to have a family and makes real economic sense. Britain is leading the world on sexual and reproductive health and women’s rights, and we have helped 8.5 million more women make an informed choice about using desperately-needed family planning services since 2012. Today’s Summit with our international partners is an exciting and important opportunity to work together to empower millions more women and girls to take control of their own lives, address unsustainable population growth and boost global prosperity.”Dr Natalia Kanem, Acting Executive Director of the United Nations Population Fund (UNFPA), said: “Together we must keep our promise to leave no woman behind. Even though family planning is one of the best investments for development and women’s empowerment, 214 million women in developing countries who want family planning still lack access to modern contraception. To meet our common goal of reaching 120 million more women and girls by 2020, we must strengthen partnerships and mobilize more funding. As we strive to achieve universal access to sexual and reproductive health and reproductive rights, we are committed to getting to zero unmet need for family planning by 2030 to ensure a better future for all.”Melinda Gates, Co-Chair of the Bill & Melinda Gates Foundation, said: “Access to contraceptives changes everything. Women are freer to work outside the home, earn an income, and contribute to the economy. Mothers and fathers can devote more resources to their kids’ health and education-setting them up for a more productive future. Multiply that by millions of families, and you see why contraceptives are one of the greatest antipoverty innovations the world has ever known-and one of the smartest investments countries can make. Through the new commitments expected today at the Family Planning Summit, we have made a bold statement that investing in family planning is crucial to building the healthier and more prosperous future we’re all working towards.”Beth Schlachter, Executive Director of FP2020, said: “We are guided by the urgency of knowing that our work affects the lives and futures of women every day. The Summit is a chance to evaluate our progress on family planning, and re-dedicate ourselves to bringing this life-saving intervention to all that need and want it.”
Advertisement And after 12 years as our correspondent in Montreal, Natasha Gargiulo will continue host and produce entertainment shows for TV and radio, and expand her lifestyle retreats to empower women.You can still catch Natasha and Erin on the show throughout the month of July, and Rick will appear until the end of August.We sincerely thank Rick, Erin and Natasha for their tremendous contributions to ET Canada, and wish them every success in their future endeavours. Advertisement LEAVE A REPLY Cancel replyLog in to leave a comment Advertisement Heading into our 13th season of ET Canada, we are making some changes to the show. It’s an emotional day as we share the news that Rick Campanelli, Erin Cebula and Natasha Gargiulo will be moving on to pursue new ventures.After 12 years as co-host, Rick will be leaving ET Canada when his contract comes to a close later this summer. We are grateful for his dedication to the show and wish him well.Following 12 years as our West Coast reporter, Erin is moving on to produce and host quality lifestyle and entertainment content for broadcast and new media in Vancouver. Login/Register With: Facebook Twitter
President Donald Trump on Friday signed a two-bill spending package that fully funds the Pentagon in fiscal 2019, lifting defense officials from the challenge of coping with short-term spending measures for the first time in 10 years. The legislation’s enactment marks a rare achievement, especially after the president one week earlier signed a three-bill package funding the department’s military construction programs in FY 2019. The defense spending bill provides $674.4 billion in discretionary budget authority and represents a $17 billion increase in DOD’s base budget funding over the FY 2018 allocation. It fully funds the administration’s requested 16,400 end-strength increase this year, including boosting active forces by 15,600 personnel, reports Military Times. … While its long-term fate remains uncertain, an immigration program which has provided a significant source of financing for base redevelopment projects has been extended through Dec. 7. The EB-5 Immigrant Investor program grants 10,000 visas annually to foreigners and their families who have invested $1 million in projects in the United States that create at least 10 jobs, or who have invested $500,000 if the money supports areas of high unemployment. Numerous questions have been raised about the program, though, and lawmakers from both parties have proposed terminating it, reports CQ.DOD photo by Michael Strasser Dan Cohen AUTHOR
Email Facebook Changing West Eighth St. To “Jimi Hendrix Way” new-yorkers-want-call-w-8th-st-jimi-hendrix-way News New Yorkers Want To Call W. 8th St. “Jimi Hendrix Way” Twitter Greenwich Village, N.Y., home of Electric Lady Studios hopes the city will dub their neighborhood with the guitarist’s legendary namePhilip MerrillGRAMMYs Oct 5, 2017 – 4:29 pm The life and art of Jimi Hendrix are touchstones that artists and fans revisit, for example Outkast’s André 3000 playing him in the 2013 biopic Jimi: All Is By My Side. Now residents and merchants on W. 8th Street in New York City’s Greenwich Village hope co-naming their street “Jimi Hendrix Way” will have the same effect on tourists and foot traffic.Comparable to the 1977 renaming of a portion of W 106th Street as Duke Ellington Boulevard by the Riverside Drive home where he composed, these Greenwich Villagers want to memorialize the magic of Electric Lady Studios, which Hendrix bought in 1968 and opened in 1970, the year of his death.Local merchant Richard Geist said, “We want to bring that magic back.”The co-naming process is still at the stage of collecting petition signatures, after which the next steps are community board support followed by a full vote of the New York City Council.The Making Of Jimi Hendrix’s Are You Experienced?
WILMINGTON, MA — Here are highlights of the Wilmington Police Log for Sunday, June 30, 2019:Police were off with a group of juveniles at Town Hall. They check OK. Parents were aware. Kids were heading to CVS. (2:06am)Police reported 10 mailboxes damaged and/or on the ground on Lawrence Street. (2:11am)Police notified DPW of two street signs missing on New Hampshire Road. (2:46am)During a check of Town Hall, a resident notified police of a silver Mongoose bike that was on site for nearly month. Police notified DPW for pick-up. (8:58am)(DISCLAIMER: This information is public information. An arrest does not constitute a conviction. Any arrested person is innocent until proven guilty.)Like Wilmington Apple on Facebook. Follow Wilmington Apple on Twitter. Follow Wilmington Apple on Instagram. Subscribe to Wilmington Apple’s daily email newsletter HERE. Got a comment, question, photo, press release, or news tip? Email email@example.com.Share this:TwitterFacebookLike this:Like Loading… RelatedPOLICE LOG for September 2: Beer Cans Found At Town Park; Spray Paint At Palmer Park; Sinkhole WorseningIn “Police Log”POLICE LOG for July 14: Unlocked Vehicles Entered Overnight; Trouble At Group Home; Street Sign & Mailbox VandalizedIn “Police Log”POLICE LOG for June 26: 2-Year-Old Locks Family Out; Co-Workers Get In Fight; Don’t Watch YouTube While DrivingIn “Police Log”
Reuters file [Representational Image]The benchmark BSE Sensex advanced over 100 points in early trade Wednesday on gains in bluechips like Maruti Suzuki, SBI, Tata Steel and ITC amid rising rupee and tumbling crude prices.Rising for the seventh straight session, the 30-share index spurted 104.05 points, or 0.29 per cent, to 36,451.13. The barometer had rallied over 1,387 points in the previous six sessions.Led by FMCG, oil and gas, PSU and realty, sectoral indices advanced up to 0.99 per cent. The NSE Nifty saw similar movement, rising up 36.60 points, or 0.34 per cent, to 10,945.30.Brokers said sustained buying by investors and foreign fund inflows, coupled with strengthening rupee against the US dollar and falling global crude oil prices, influenced trading sentiment here.Negative Asian cues on a likely Federal Reserve interest rate hike, limited the gains here, traders said. The rupee breached the 70 mark by surging 50 paise to trade at 69.94 against the dollar in early trade at the interbank forex market.Prominent gainers were Asian Paint, Axis Bank, ITC, Maruti Suzuki, SBI, HDFC, Yes Bank, Bharti Airtel, HUL, Tata Steel, Bajaj Auto, L&T, RIL and HDFC Bank, rising up to 1.70 per cent.However, Infosys emerged worst Sensex performer, falling 1.20 per cent, followed by Vedanta, TCS, Tata Motors and Sun Pharma. Shares of aviation companies rose after crude prices hit a 14-month low. InterGlobe Aviation, SpiceJet and Jet Airways rallied up to 5 per cent.Stocks of oil marketing companies like IOC, HPCL, BPCL and OIL too gained up to 1.43 per cent. Brent crude, an international benchmark, dropped 4.2 per cent lower to USD 57.07 per barrel Tuesday, a 14-month low.Meanwhile, foreign institutional investors (FIIs) bought shares to the tune of Rs 144.76 crore Tuesday, while domestic institutional investors (DIIs) sold shares worth a net of Rs 182.60, provisional data showed. Elsewhere in Asia, Japan’s Nikkei fell 0.43 per cent, Shanghai Composite Index shed 0.25 per cent, while Hong Kong’s Hang Seng rose 0.10 per cent.The US Dow Jones Industrial Average ended 0.35 per cent higher Tuesday.-PTI
Implementing the single-molecule DNA navigator. a) Schematic illustration of the PSEC system. An acyclic connected graph (i.e. a tree) is constructed on a rectangular DNA origami substrate of 100 x 70 nm^2. T1 and T2 are two types of fuels driving the PSEC on the tree. Initiator I was used to trigger the initiation of the PSEC from the entrance vertex ENT. b) propagation mechanism of the PSEC system. Initiator I recognizes and opens the T1ent hairpin at vertex ENT. The opened hairpin then captures and opens a T2 hairpin from the environment to start the cascade. c) A straight line paved by PSEC and visualized with DNA-PAINT. d) Four paved digits of “2017” imaged using atomic force microscopy (AFM). The T1 hairpins at the corner points were modified to prevent unwanted spans. The arrows indicate the direction of propagation. Credit: Nature Materials, doi: 10.1038/s41563-018-0205-3. © 2018 Science X Network Tying the knot: New DNA nanostructures , Science In the present study by Chao et al, the same basic principle of the HCR reaction scheme was used in a different computational context to develop a single-molecule DNA-navigator system. The platform explored all possible paths through a tree graph designed on an origami structure as a simply connected maze without cyclic paths. Such DNA origami structures are information-bearing nanostructures by nature with well-defined nanoscale geometry. The maze could be explored by proximal strand exchange cascade (PSEC) based on hybridization chain reactions. The researchers demonstrated that a system with a large number of single-molecule DNA navigators could collectively conduct parallel depth-first search (PDFS) on the tree to efficiently perform maze solving within 2-D origami. Initially the researchers conducted studies to test the PSEC design. Journal information: Nature Materials Citation: Solving mazes with single-molecule DNA navigators (2018, November 16) retrieved 18 August 2019 from https://phys.org/news/2018-11-mazes-single-molecule-dna.html More information: Jie Chao et al. Solving mazes with single-molecule DNA navigators, Nature Materials (2018). DOI: 10.1038/s41563-018-0205-3Renjun Pei et al. Training a molecular automaton to play a game, Nature Nanotechnology (2010). DOI: 10.1038/nnano.2010.194S. M. Douglas et al. A Logic-Gated Nanorobot for Targeted Transport of Molecular Payloads, Science (2012). DOI: 10.1126/science.1214081 Kinetics of the process were investigated at the single-molecule level in the study using time-resolved total internal reflection fluorescence microscopy (TIRF) in a setup with a prescribed starting point (P0) and five intermediate steps (P1-P5). Fluorescence in the setup was quenched using fluorescence resonance energy transfer (FRET), and the cascade was observed in real time by recording fluorescence signals continuously. The average speed of propagation was recorded to be 2.46 nm per minute, propagation across the straight line (54.4 nm) took approximately 22 minutes. The scientists then constructed the main model maze with 10 vertices that included an entrance vertex A and an exit vertex J, three junctions (B, D, E) an intermediate vertex (I) and four dead ends (C, F, G, H). Each path of the maze that was equivalent to a tree with 10 vertices was investigated using DNA investigators starting at root A. The PSEC reactions produced a mixture of various paths on the maze, confirmed with AFM. Each individual PSEC could progress on one of the five possible paths. Statistical analysis of the length distribution showed that the measured paths coincided well with the values predicted. To prevent the navigators from propagating through a wrong path with dead ends, the scientists designed a streptavidin-biotin tag-based method to selectively eliminate inaccurate path navigation. Only the correct path (PABDIJ) was followed in the maze therefore. The computational context used in the study allowed exploration of paths through tree graphs defined on the origami. The autonomous path explored by the DNA navigators proceeded unidirectionally and irreversibly, turning at junctions and corners on the origami platform as they were designed to. The design enabled parallel depth first search (PDFS) allowing each DNA navigator to individually explore any one of the paths through the given graph at a defined speed, greater than that previously achieved. The main advantage of the described biomolecular computer schemes in comparison to conventional electronic computing is they can be interfaced directly with biologically relevant processes. As a result, the scientists envision translational biomedical sensing and decision-making platforms with DNA origami and single-molecule diagnostics using decision trees. Future applications will also include simple sensors or those coupled to a molecular actuator to trigger downstream molecular cascades. , Nature Nanotechnology Pathfinding operations with DNA navigators make use of a localized strand exchange cascade process initiated at a unique trigger site on the origami platform. Automatic progression along paths is enabled by DNA hairpins containing a universal traversal sequence. By design, each single-molecule navigator can autonomously explore any of the possible paths through a 10-vertex rooted tree constructed in the study. The mazes were equivalent to a tree with an entrance at the root and an exit through one of the leaves. The study conducted by Jie Chao and co-workers resulted in exploring all paths taken by the DNA navigators to extract a specific solution path that connected a given pair of start and end vertices in the maze. As a result, the solution path was laid plainly on the origami platform and illustrated using single-molecule imaging. The approach is now published in Nature Materials, detailing the realization of molecular materials with embedded biomolecular computational functions to operate at the level of the single-molecule with potential to engineer intelligent nanorobots for future applications in industry and medicine.Sophisticated molecular tools were used in the past to create molecular machines that convert chemical, photonic or electric energy into rotary or linear movements at the nanoscale. For instance, Brownian motion at the nanoscale can be controllably converted into directed movements within DNA-based nanomachines using DNA hybridization reactions. Such DNA-based machines operate autonomously by following an embedded ‘molecular program’ pre-designed as a cascade reaction manually triggered via an external stimulus for each step of the operation. The focus of the field has progressively shifted to actualize DNA-based logic circuits using aptamers and DNAzymes to design molecular logic gates. For example, in 2006, Stojanovic and co-workers integrated more than 100 DNA logic gates to engineer an automation calledMAYA-II to play a game of Tic-Tac-Toe. Preceding studies demonstrated an enzyme free computing system based on hybridization chain reactions (HCR) to create logic-gates and logic circuits for more robust and efficient performance than the original systems. Single-molecule DNA navigators for maze-solving. a) Schematic illustration of magnetic bead-based selection. Exit vertex J is labelled with biotin to enable differentiation between the correct and wrong paths. Only if the PSEC reaches the correct exit J, would the biotin-modified T1exit-B strand be released. All wrong paths could be captured and removed by the Streptavidin-modified magmatic beads therefore. b) Details of the release of biotin modification at the exit J by the PSEC. c) AFM characterization of the correct solution after selection. The remaining structures all showed the correct solution path PABDIJ. d) Single-molecule and class-averaged DNA-PAINT characterization of the correct solution after selection. Credit: Nature Materials, doi: 10.1038/s41563-018-0205-3. Explore further The proximal strand exchange cascade (PSEC) system (working principle of the DNA navigator) was facilitated on a rectangular origami substrate made of three components, which included the physical implementation of a tree graph, full strands and an initiator strand. Vacant areas without staple extensions corresponded to walls in the maze, preventing propagation of the strand exchange cascade. The entrance and exit were defined and denoted as ENT and EXIT respectively. In the second component, two types of DNA hairpins, T1 and T2, were used as fuels to drive the PSEC on the tree graph. The two hairpins coexisted metastably in solution to hybridize and fuel the PSEC process with free energy . By design, information only propagated through the network in the presence of an initiator (Initiator I). Upon addition of initiator I, PSEC was conducted and observed using atomic force microscopy (AFM). To visualize an established formation, the scientists enabled DNA-navigator-based formation of the number 2017 as a proof-of-principle. Another technique known as DNA-PAINT was employed as a single-molecule, super-resolution imaging technique to reveal molecular features at the nanoscale to further substantiate the PSEC-based path paving process. The on-origami PSEC was highly specific, without intra- or inter-origami crosstalk. The field of intelligent nanorobotics is based on the great promise of molecular devices with information processing capabilities. In a new study that supports the trend of DNA-based information carriers, scientists have engineered a DNA navigator system that can perform single-molecule, parallel, depth-first search operations on a two-dimensional origami platform. Single-molecule characterization of PSEC kinetics. a) A straight line in the middle of the DNA origami was used as the test bed. Vertex P0 is the starting point and vertices P1-P5 are intermediate points. b) Details of the design of six parallel tests to measure the kinetics with time-resolved TIRF. T2 labelled with BHQ2 was used to quench T1 labelled with Cy3, assembling a kinetic profile at each step. The illustrated example shows the mechanism of quenching. c) Example TIRF images show the fluorescence changing with time across the six parallel tests from P0 to P5. d) Typical single-molecule fluorescence traces used to monitor quenching events occurring at vertices P0 to P5. e) Scatter plots showing statistical analysis of the length distribution per path. Credit: Nature Materials, doi: 10.1038/s41563-018-0205-3. This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. The PSEC-driven graph traversal on a maze. a) A maze design with 10 vertices. Arrows indicate the entrance vertex A and exit vertex J. b) The maze is equivalent to a rooted tree with 10 vertices. The entrance vertex A corresponds to the root of the tree. c) An AFM image showed the result of a transversal experiment generating all possible paths. In this DNA computing implementation of a PDFS algorithm, a vast number of PSEC events simultaneously occurred to realize the graph traversal on the maze. PSEC ending at the exit or deadends were highlighted in red circles. Invalid structures were highlighted with white circles. d) Typical paths found in the mixture seen from left to right. Only PABDIJ was the correct solution to the maze. e) Scatter plots showing the statistical analysis of the length distribution for each path. Credit: Nature Materials, doi: 10.1038/s41563-018-0205-3.
Just two days ago, Perfecto, a Perforce Software company introduced Perfecto Codeless, an AI-driven codeless testing solution for eliminating the need for coding skills in the dev testing process. Perfecto Codeless will help the development teams to automate the process of writing test scripts as it comes with machine learning (ML) capabilities. The scripts will be allowed to run continuously and fix themselves without disrupting operations. Eran Yaniv, Founder, and CEO at Perfecto wrote to us in an email, “Across our customer base, the number one cause of automation failure is scripting issues. This a huge barrier for achieving good test automation and teams making their way towards continuous testing. With the introduction of Perfecto Codeless, we are harnessing the power of machine learning to offer the next generation of codeless automation testing capabilities. By eliminating the need to write and maintain test scripts, teams save time and can focus on more complex tasks.” The development teams have the coding skills for writing Selenium or Appium scripts but it is better if their time is spent on product development and innovation. Perfecto Codeless comes with tools that help the teams to quickly generate quality test scripts and maintain them. Features of Perfecto Codeless Smart capabilities to maintain scripts Perfecto’s ML capabilities address object maintenance issues within the code. If there is a need for deleting, moving or changing the code, Perfecto Codeless makes it happen agnostically without delaying the process. Interconnected components All the components of the testing process are connected right from creation to execution and analysis. Codeless automation in the cloud Perfecto Codeless provides codeless test automation in the cloud that allows teams to manage the pace and demands that come with test automation. Perfecto Codeless also provides the flexibility, performance, and scalability needed to ensure quality throughout the SDLC. Eran Kinsbruner, Chief Evangelist at Perfecto wrote to us in an email, “In recent years, codeless test automation has become a top priority for testers, as well as the developers that aim to expedite their test creation and maximize testing reliability. These professionals are looking at codeless as a preferred solution to embed into their testing responsibilities. Perfecto Codeless will take DevOps to the next level, relieving testers and developers of the time-intensive responsibility of coding and giving them time back to focus on product development and innovation to help accelerate the software delivery lifecycle (SDLC) for their business.” Read Next Rachel Batish’s 3 tips to build your own interactive conversational app DeepMind researchers provide theoretical analysis on recommender system, ‘echo chamber’ and ‘filter bubble effect’ Waymo to sell its 3D perimeter LIDAR sensors to companies outside of self-driving