Cleveland Cavaliers star LeBron James took issue Monday with New York Knicks President Phil Jackson’s use of the term “posse,” leading to a lively discussion on Twitter.James’ issue stems from a statement Jackson made to ESPN Nov. 14, when he referred to the NBA player’s business partners and friends as a “posse.”“When LeBron was playing with the [Miami] Heat, they went to Cleveland and he wanted to spend the night,” Jackson told ESPN. “They don’t do overnights. Teams just don’t. So now [Heat coach Erik] Spoelstra has to text [Heat president Pat] Riley and say, ‘What do I do in this situation?’ And Pat, who has iron-fist rules, answers, ‘You are on the plane. You are with this team.’ You can’t hold up the whole team because you and your mom and your posse want to spend an extra night in Cleveland.“I always thought Pat had this really nice vibe with his guys,” Jackson continued. “But something happened there where it broke down. I do know LeBron likes special treatment. He needs things his way.”When asked for his response, James told ESPN he had “no relationship at all” to the former Chicago Bulls coach.“I had nothing but respect for him as a coach for what he was able to do,” he noted.But what Twitter latched onto was James’ issue with the word “posse.”“To use that label – and if you read the definition of what the word ‘posse’ is – it’s not what I’ve built over my career. It’s not what I stand for,” the athlete said. “It’s not what my family stands for. And I believe the only reason he used that word is because he sees a young African-American trying to make a difference.”In response, D.J. Andrews called for Jackson to lose his position.Somebody gotta hack Phil Jackson email and get him outta here too— D.J. Andrews (@DJDarrel3) November 15, [email protected] agreed with James but told him to respect the coach who led the Lakers for more than a decade.Ok Lebron I agree with you about Phil Jackson , but you better respect the greatest coach ever!— Jesus (@KobeTruthNlight) November 15, 2016Jammy Jam also sided with James. He referenced Jackson’s history of speaking on issues he was not originally involved in. The retired coach previously put his two cents’ worth in on a 2012 feud between former Lakers players Dwight Howard and Shaquille O’Neal.Idc I’m team Bron Bron, I don’t like Phil Jackson anyway. He be talking shit and acting up but supposed to be all about zen lol— Jammy Jam (@OfficialJammy) November 15, 2016But some users defended Jackson. They backed away from James’ longtime business associate Maverick Carter’s assertion that Jackson was disrespectful.All the hard work,effort and achievement and @PhilJackson11 still calls us a “posse”every step you take they remind you, you ghetto” @S_C_ pic.twitter.com/OPsPvAvFoM— Maverick Carter (@mavcarter) November 14, 2016But Fire Rich Cho checked Carter on the word’s [email protected] @PhilJackson11 @S_C_ lemme finish it for you. Look at the final definition and stop being so sensitive. pic.twitter.com/4KB4siTRmU— Fire Rich Cho (@FireRichCho) November 15, 2016Carter later clarified his stance, noting he didn’t call Jackson racist.Let me be clear I’m not saying @PhilJackson11 is racist,, I’m calling out his disrespectful language— Maverick Carter (@mavcarter) November 15, 2016Meanwhile, Marquisha Jeng explained the origins of the word “posse” before deciding Jackson “meant no harm.”’Posse’ is derived from the word ‘posse comitatus’, which is a legal term defining power or authority. Phil Jackson meant no harm…— Marquisha Jeng (@_melaninhippie_) November 15, 2016Truth Seeker questioned why James felt disrespected by Jackson while he “went back to slave master Dan Gilbert.” The statement refers to Gilbert’s open letter in which he blasted James for leaving the Heat in 2010. During the ensuing fallout, Rev. Jesse Jackson told the Associated Press Gilbert saw James as an enslaved African who escaped from his slavemaster.“His feelings of betrayal personify a slave master mentality,” Jackson said. “He sees LeBron as a runaway slave. This is an owner/employee relationship – between business partners – and LeBron honored his contract.”I mean I’m black power and everything, but for LeBron to say Phil Jackson disrespected him and he went back to slave master Dan Gilbert. Smh— Truth Seeker (@xfilestrustno1) November 15, 2016Additionally, Checo said he didn’t believe the Knicks’ president was racist.I don’t think Phil Jackson ment for his statement to come off as racist. If he was then he made the WORST career choice. #LeBronJames #NBA— Checo (@ChecoRepublic) November 15, 2016
Implementing the single-molecule DNA navigator. a) Schematic illustration of the PSEC system. An acyclic connected graph (i.e. a tree) is constructed on a rectangular DNA origami substrate of 100 x 70 nm^2. T1 and T2 are two types of fuels driving the PSEC on the tree. Initiator I was used to trigger the initiation of the PSEC from the entrance vertex ENT. b) propagation mechanism of the PSEC system. Initiator I recognizes and opens the T1ent hairpin at vertex ENT. The opened hairpin then captures and opens a T2 hairpin from the environment to start the cascade. c) A straight line paved by PSEC and visualized with DNA-PAINT. d) Four paved digits of “2017” imaged using atomic force microscopy (AFM). The T1 hairpins at the corner points were modified to prevent unwanted spans. The arrows indicate the direction of propagation. Credit: Nature Materials, doi: 10.1038/s41563-018-0205-3. © 2018 Science X Network Tying the knot: New DNA nanostructures , Science In the present study by Chao et al, the same basic principle of the HCR reaction scheme was used in a different computational context to develop a single-molecule DNA-navigator system. The platform explored all possible paths through a tree graph designed on an origami structure as a simply connected maze without cyclic paths. Such DNA origami structures are information-bearing nanostructures by nature with well-defined nanoscale geometry. The maze could be explored by proximal strand exchange cascade (PSEC) based on hybridization chain reactions. The researchers demonstrated that a system with a large number of single-molecule DNA navigators could collectively conduct parallel depth-first search (PDFS) on the tree to efficiently perform maze solving within 2-D origami. Initially the researchers conducted studies to test the PSEC design. Journal information: Nature Materials Citation: Solving mazes with single-molecule DNA navigators (2018, November 16) retrieved 18 August 2019 from https://phys.org/news/2018-11-mazes-single-molecule-dna.html More information: Jie Chao et al. Solving mazes with single-molecule DNA navigators, Nature Materials (2018). DOI: 10.1038/s41563-018-0205-3Renjun Pei et al. Training a molecular automaton to play a game, Nature Nanotechnology (2010). DOI: 10.1038/nnano.2010.194S. M. Douglas et al. A Logic-Gated Nanorobot for Targeted Transport of Molecular Payloads, Science (2012). DOI: 10.1126/science.1214081 Kinetics of the process were investigated at the single-molecule level in the study using time-resolved total internal reflection fluorescence microscopy (TIRF) in a setup with a prescribed starting point (P0) and five intermediate steps (P1-P5). Fluorescence in the setup was quenched using fluorescence resonance energy transfer (FRET), and the cascade was observed in real time by recording fluorescence signals continuously. The average speed of propagation was recorded to be 2.46 nm per minute, propagation across the straight line (54.4 nm) took approximately 22 minutes. The scientists then constructed the main model maze with 10 vertices that included an entrance vertex A and an exit vertex J, three junctions (B, D, E) an intermediate vertex (I) and four dead ends (C, F, G, H). Each path of the maze that was equivalent to a tree with 10 vertices was investigated using DNA investigators starting at root A. The PSEC reactions produced a mixture of various paths on the maze, confirmed with AFM. Each individual PSEC could progress on one of the five possible paths. Statistical analysis of the length distribution showed that the measured paths coincided well with the values predicted. To prevent the navigators from propagating through a wrong path with dead ends, the scientists designed a streptavidin-biotin tag-based method to selectively eliminate inaccurate path navigation. Only the correct path (PABDIJ) was followed in the maze therefore. The computational context used in the study allowed exploration of paths through tree graphs defined on the origami. The autonomous path explored by the DNA navigators proceeded unidirectionally and irreversibly, turning at junctions and corners on the origami platform as they were designed to. The design enabled parallel depth first search (PDFS) allowing each DNA navigator to individually explore any one of the paths through the given graph at a defined speed, greater than that previously achieved. The main advantage of the described biomolecular computer schemes in comparison to conventional electronic computing is they can be interfaced directly with biologically relevant processes. As a result, the scientists envision translational biomedical sensing and decision-making platforms with DNA origami and single-molecule diagnostics using decision trees. Future applications will also include simple sensors or those coupled to a molecular actuator to trigger downstream molecular cascades. , Nature Nanotechnology Pathfinding operations with DNA navigators make use of a localized strand exchange cascade process initiated at a unique trigger site on the origami platform. Automatic progression along paths is enabled by DNA hairpins containing a universal traversal sequence. By design, each single-molecule navigator can autonomously explore any of the possible paths through a 10-vertex rooted tree constructed in the study. The mazes were equivalent to a tree with an entrance at the root and an exit through one of the leaves. The study conducted by Jie Chao and co-workers resulted in exploring all paths taken by the DNA navigators to extract a specific solution path that connected a given pair of start and end vertices in the maze. As a result, the solution path was laid plainly on the origami platform and illustrated using single-molecule imaging. The approach is now published in Nature Materials, detailing the realization of molecular materials with embedded biomolecular computational functions to operate at the level of the single-molecule with potential to engineer intelligent nanorobots for future applications in industry and medicine.Sophisticated molecular tools were used in the past to create molecular machines that convert chemical, photonic or electric energy into rotary or linear movements at the nanoscale. For instance, Brownian motion at the nanoscale can be controllably converted into directed movements within DNA-based nanomachines using DNA hybridization reactions. Such DNA-based machines operate autonomously by following an embedded ‘molecular program’ pre-designed as a cascade reaction manually triggered via an external stimulus for each step of the operation. The focus of the field has progressively shifted to actualize DNA-based logic circuits using aptamers and DNAzymes to design molecular logic gates. For example, in 2006, Stojanovic and co-workers integrated more than 100 DNA logic gates to engineer an automation calledMAYA-II to play a game of Tic-Tac-Toe. Preceding studies demonstrated an enzyme free computing system based on hybridization chain reactions (HCR) to create logic-gates and logic circuits for more robust and efficient performance than the original systems. Single-molecule DNA navigators for maze-solving. a) Schematic illustration of magnetic bead-based selection. Exit vertex J is labelled with biotin to enable differentiation between the correct and wrong paths. Only if the PSEC reaches the correct exit J, would the biotin-modified T1exit-B strand be released. All wrong paths could be captured and removed by the Streptavidin-modified magmatic beads therefore. b) Details of the release of biotin modification at the exit J by the PSEC. c) AFM characterization of the correct solution after selection. The remaining structures all showed the correct solution path PABDIJ. d) Single-molecule and class-averaged DNA-PAINT characterization of the correct solution after selection. Credit: Nature Materials, doi: 10.1038/s41563-018-0205-3. Explore further The proximal strand exchange cascade (PSEC) system (working principle of the DNA navigator) was facilitated on a rectangular origami substrate made of three components, which included the physical implementation of a tree graph, full strands and an initiator strand. Vacant areas without staple extensions corresponded to walls in the maze, preventing propagation of the strand exchange cascade. The entrance and exit were defined and denoted as ENT and EXIT respectively. In the second component, two types of DNA hairpins, T1 and T2, were used as fuels to drive the PSEC on the tree graph. The two hairpins coexisted metastably in solution to hybridize and fuel the PSEC process with free energy . By design, information only propagated through the network in the presence of an initiator (Initiator I). Upon addition of initiator I, PSEC was conducted and observed using atomic force microscopy (AFM). To visualize an established formation, the scientists enabled DNA-navigator-based formation of the number 2017 as a proof-of-principle. Another technique known as DNA-PAINT was employed as a single-molecule, super-resolution imaging technique to reveal molecular features at the nanoscale to further substantiate the PSEC-based path paving process. The on-origami PSEC was highly specific, without intra- or inter-origami crosstalk. The field of intelligent nanorobotics is based on the great promise of molecular devices with information processing capabilities. In a new study that supports the trend of DNA-based information carriers, scientists have engineered a DNA navigator system that can perform single-molecule, parallel, depth-first search operations on a two-dimensional origami platform. Single-molecule characterization of PSEC kinetics. a) A straight line in the middle of the DNA origami was used as the test bed. Vertex P0 is the starting point and vertices P1-P5 are intermediate points. b) Details of the design of six parallel tests to measure the kinetics with time-resolved TIRF. T2 labelled with BHQ2 was used to quench T1 labelled with Cy3, assembling a kinetic profile at each step. The illustrated example shows the mechanism of quenching. c) Example TIRF images show the fluorescence changing with time across the six parallel tests from P0 to P5. d) Typical single-molecule fluorescence traces used to monitor quenching events occurring at vertices P0 to P5. e) Scatter plots showing statistical analysis of the length distribution per path. Credit: Nature Materials, doi: 10.1038/s41563-018-0205-3. This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. The PSEC-driven graph traversal on a maze. a) A maze design with 10 vertices. Arrows indicate the entrance vertex A and exit vertex J. b) The maze is equivalent to a rooted tree with 10 vertices. The entrance vertex A corresponds to the root of the tree. c) An AFM image showed the result of a transversal experiment generating all possible paths. In this DNA computing implementation of a PDFS algorithm, a vast number of PSEC events simultaneously occurred to realize the graph traversal on the maze. PSEC ending at the exit or deadends were highlighted in red circles. Invalid structures were highlighted with white circles. d) Typical paths found in the mixture seen from left to right. Only PABDIJ was the correct solution to the maze. e) Scatter plots showing the statistical analysis of the length distribution for each path. Credit: Nature Materials, doi: 10.1038/s41563-018-0205-3.
You decide what you watch. That is the funda behind Humour Me’s Improv Nights. Here audience is the king as artistes will improvise on the spot in order to entertain you. Their one-hour performance on Sunday saw an entertaining stand-up performance where they improvised on the spot, spontaneously without any pre-written script. The audience drove the theme of each segment of the show. Humour Me is a group of four performers and one host. The artistes performing at Improv Nights are Dhruv Sachdeva, Clifford Alfonso, Andrew Hoffland, Pranay Manchanda and Shantanu Anam. Also Read – ‘Playing Jojo was emotionally exhausting’Dhruv Sachdeva takes up the role of the host and is also the founder of Humour Me and has been in the entertainment world for the past 10 years. He started his acting career with the role of Scar in the musical Circle of Life which is an adaptation of the Broadway musical The Lion King’ Last year, Dhruv produced and acted in the production The Character of a Happy Life. And most recently, wrote, directed and acted in an original musical theatre production Also Read – Leslie doing new comedy special with NetflixDinner for Pricks, under the Humour Me banner.So the audience gives them a situation or a scene to enact, and they perform it on the spot — with sound effects and even mimicry et al in place.‘We basically perform as per the demand of the audience which includes different dance forms like rap, conventional etc and various music genres too,’ said Dhruv.Clifford started years back with the musical production Noah’s Ark. Andrew Hoffland, oldest of all the performers, works as a teacher and has been singing and acting in various theatrical and musical productions. Pranay Manchanda has been an actor for over 15 years, and has worked in theatre.Shantanu Anam has worked as is an actor from the age of 16, and has acted and directed several theatre productions. ‘We are here to deliver happiness, not shoes. If we don’t stick in your mind, we consider we have have failed,’ said Dhruv. Fair enough!DETAILAt: Lure Switch, Lado Sarai when: 23 December